The R&D department of Biopulse Biotech, has built several hardware prototypes, to test different configurations of plant exosomes uploads during the Cargo Loading tests. For example, different universal bases were created to accommodate cuvettes of various kinds, and other complementary accessories that helped our scientists to measure certain parameters during loading tests, such as temperature and electrical impedance during applied pulses. In the laboratories we have loaded different substances inside the plant exosomes, with excellent results in terms of retention, especially when compared to the tests carried out on liposomes. The molecules of various nature were loaded into isolated suspended and resuspended plant nanovesicles analyzed with Nanoparticle Tracking Analysis using Nanosight for the evaluation of concentration and size distribution.
All the prototypes and evolutions of the BioDischarger electroporators® built by Biopulse Biotech, have led our research group to conclude the first settings of the electroporators intended for the market. Normally, the basic electrical protocol provides for the first electrical impulse with the function of destabilizing the membrane making it permeable, and the subsequent train of electrical pulses to facilitate the transmembrane ionic (or molecular) transit. All the presets of electrical protocols applicable to Cargo Loading technology are completely expandable and customizable in terms of duration and electrical voltage. Our research group has allowed us to install a broth of the BioDischarger electroporator®, a series of durations and voltages that take into account the different sizes and stability of exosomal membranes, which have been developed on the basis of experiences of conveying molecules inside cells.